Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 1. In a crude membrane-enriched fraction, insulin-dependent diabetes results in no change in cardiac GLUT12 protein content, despite decreased cardiac GLUT4. Top panel: Western blot of cardiac muscle from healthy (Con) and type 1 diabetic (Dx) mice. Total calsequestrin (CASQ) protein content was used as a loading control. Bottom panel: Mean±SE total GLUT protein content in a crude membrane-enriched fraction, (n=5–10/group), *Pb0.05 vs. control group.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Membrane, Western Blot, Control

Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 4. Insulin stimulation causes significant translocation of GLUT4, but not GLUT12, to cell peripherals, as visualized by confocal laser scanning microscopy. Adult rat cardiac myocytes were incubated without (A, C) or with insulin (B, D, 100 μU/ml) for 30 min prior to immunofluorescent staining. Results were analyzed from three independent exper- iments with at least 70 cells/treatment. Scale bar: 10 μm.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy, Incubation, Staining